| 講師 |
ヌシャテル大学マイクロテクノロジー研究所 Dr. Sander Koster |
| 題目 |
Microfluidics: new tools to study the response of cells to chemicals |
| 日時 |
2004年2月24日(火) 14:00〜15:30 |
| 場所 |
生産技術研究所・第四会議室 |
| 講演要旨 |
The first part of the talk will deal with a general overview of the
microTAS
activities performed at the Institute of Microtechnology. These
activities
include the use of trapped freely moving beads in recirculating flows,
heterogeneous immunoassays, on-chip heating/cooling and contactless
conductivity detection.
In the second part of the talk a novel method is presented where living
nerve cells are cultured in a cultivation chamber. The goal is to use
drugs
to stimulate individual groups of living cells, arranged in an nxm
array on
a surface. Such experiments are increasingly important in applications
where
a large number of analyses are required, such as drug discovery,
environmental monitoring and neural computing. A microfluidic system
containing a 50-μL chamber etched in silicon with KOH is presented for
the
cultivation of living cells. Nutrients are introduced through a 1-mm
ultrasonically drilled hole in the Pyrex cover-plate to keep the nerve
cells
alive. A novel concept for the delivery of drugs to cells is presented,
where drugs will be delivered to the cells through 20-μm holes drilled
with
an excimer laser in the same Pyrex coverplate. The holes are connected
with
a microfluidic network of channels through which drugs can be pumped
individually, as has been demonstrated with slime mold (Physarum
polycephalum), a multi-nucleated single cell, that was grown in the flow
cell. Ethanol was delivered in a spatially resolved manner to the
growing
slime mold affecting the viability of only part of the organism. |
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